ASTM D4454-1985(2009) 用显微镜进行水生系统中细菌总量和(呼吸时)呼出的细菌的同步计数的标准试验方法

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【英文标准名称】:StandardTestMethodforSimultaneousEnumerationofTotalandRespiringBacteriainAquaticSystemsbyMicroscopy
【原文标准名称】:用显微镜进行水生系统中细菌总量和(呼吸时)呼出的细菌的同步计数的标准试验方法
【标准号】:ASTMD4454-1985(2009)
【标准状态】:现行
【国别】:美国
【发布日期】:1985
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:D19.24
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:
【英文主题词】:Water-formeddeposits;Bacteria/bacterialcontrol--water;Epifluorescencemicroscopy;Fluorescence--wateranalysis;Microscopicexamination--water;Pathogens--water
【摘要】:Measurementofbacterialdensitiesisgenerallythefirststepinestablishingarelationshipbetweenbacteriaandotherbiochemicalprocesses.Itisknownthattheclassicalplatecountprocedureunderestimatesbacterialdensitieswhiletheepifluorescencedirectmicroscopicproceduremoreaccuratelydepictsthetotalnumbersofnonviableordormantandviablecellsinawatersample.Theacridine-orangeINT-formazanreductiontechniqueprovidesinformationonthetotalconcentrationsofbacteriaaswellasthatproportionwhichareactivelyrespiringandthusinvolvedindegradativeprocesses.Theacridine-orangeINT-formazanreductiontechniqueisbothquantitativeandprecise.Thisprocedureisidealforenumeratingbothpelagicandepibenthicbacteriainallfreshwaterandmarineenvironments.Theprocesscanbeemployedinsurveystudiestocharacterizethebacteriologicaldensitiesandactivitiesofenvironmentalwaters.1.1Thistestmethodcoversthedetectionandenumerationofaquaticbacteriabytheuseofanacridine-orangeepifluorescencedirect-microscopiccountingprocedure.Thistestmethodisapplicabletoenvironmentalwatersandpotablewaters.1.2Certaintypesofdebrisandothermicroorganismsmayfluoresceinacridine-orangestainedsmears.1.3Theproceduredescribedrequiresatrainedmicrobiologistortechnicianwhoiscapableofdistinguishingbacteriafromotherfluorescingbodiesonthebasisofmorphologywhenviewedathighermagnifications.1.4Useofbrightlightpermitsdifferentiationofsinglebacteriawherereducedformazanisdepositedatthepolarends.1.5Approximately104cells/mLarerequiredfordetectionbythistestmethod.1.6Minimalcellsizewhichallowsthedetectionofformazandepositsisrepresentedbybacteriaof0.4x03BC;m.21.7ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.8Thisstandarddoesnotpurporttoaddressthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:Z16
【国际标准分类号】:07_100_20
【页数】:3P.;A4
【正文语种】:英语